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ARPN Journal of Science and Technology >> Volume 7, Issue 1, January 2017

ARPN Journal of Science and Technology


Vulnerability of HL-60 Cells towards TGHQ-Mediated Apoptosis during the Cell Cycle

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Author Hashimul Ehsan
ISSN 2225-7217
On Pages 982-990
Volume No. 2
Issue No. 10
Issue Date December 01, 2012
Publishing Date December 01, 2012
Keywords PS externalization, TGHQ, FCM analysis, checkpoint, apoptosis



Abstract

TGHQ, a hematotoxic metabolite of benzene, depletes intracellular glutathione and induces rapid ROS-dependent apoptosis in HL-60 cells. To determine the influence of the cell cycle on TGHQ induced apoptosis, we investigated synchronized and asynchronous cells by biochemical and flow cytometric analysis. Synchronized HL-60 cells were more sensitive to TGHQ induced apoptosis than their asynchronous counterparts. Synchronized cells underwent TGHQ- induced apoptosis in as little as 2 hours, compared to 6 hours in asynchronous cell culture. S-phase cells exposed to TGHQ initiate apoptosis as early as 40 minutes after treatment and G2-M cells were less sensitive to PS externalization compared to S-phase cells. The proteosome inhibitor MG 132 induce apoptosis but did not potentate TGHQ mediated apoptosis in ROS induced HL-60 cells.


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2012 ARPN Publishers