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ARPN Journal of Science and Technology >> Volume 7, Issue 1, January 2017

ARPN Journal of Science and Technology


Isolation and Cytotoxic Effect of Escherichia coli on Vero cells And Kidney Cells of Bovine

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Author Ismail M
ISSN 2225-7217
On Pages 482-484
Volume No. 3
Issue No. 5
Issue Date June 01, 2013
Publishing Date June 01, 2013
Keywords Ecoli, Cytotoxic, Vero cells and cells of kidney.



Abstract

The present study was aimed to isolate the Escherichia coli from different human infection and then study its cytotoxic effect on vero cells and kidney cells of bovine. 40 samples were collected as follows: 20 isolate samples from urine, 4 isolate samples from eye swabs, 5 isolate samples from wound swabs and 11 isolate samples from stool specimen. All isolate samples were fermented glucose, maltose, sorbitol and raffinose. 84% of isolate sample were fermented sucrose, 60% of isolate samples were positive to Indole test, 80% of isolate samples were negative to urease test and 70% of isolate samples were motile. All isolate samples were negative to oxidase test, Voges-Proskaur test and H2S, but they were positive to catalase test and MR test. 10 strain of E coli were selected according to their biochemical test and then investigate its cytotoxic activity on Vero cells and kidney cells of bovine. The results were indicated 1o strain of E coli had produced cytotoxic activity on the kidney cells of bovine, but they did not produce cytotoxic activity on the Vero cells.


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