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ARPN Journal of Science and Technology >> Volume 7, Issue 1, January 2017

ARPN Journal of Science and Technology


Quantitative Analysis of Ciprofloxacin Sodium Chloride Pharmaceutical Infusions Using Ultraviolet-visible Spectroscopy

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Author Affo, W., Mensah-Brown, H., Awuku, J. F., Markwo, A
ISSN 2225-7217
On Pages 296-300
Volume No. 3
Issue No. 3
Issue Date April 01, 2013
Publishing Date April 01, 2013
Keywords Absorbance, ciprofloxacin, fluoroquinolone, ultraviolet-visible spectroscopy



Abstract

This work was undertaken to develop a rapid and low cost ultraviolet-visible spectrophotometric method for determining the amount of ciprofloxacin in ciprofloxacin sodium chloride pharmaceutical infusions. Ultraviolet-visible spectrophotometric analysis was performed at a pre-determined maximum wavelength of 326 nm with water as diluent and 0.018 mg/ml sodium chloride solution as blank. The method was validated for linearity, accuracy, precision and reproducibility. The method was used to estimate the amount of ciprofloxacin in four infusion samples on the Ghanaian market. The regression data for the calibration curve exhibited a good linear relationship (R2 = 0.999) having a regression equation, y =74.554 x + 0.019 over a concentration range of 0.002 - 0.012 mg/ml. The amount of ciprofloxacin in samples A, B, C and D were 216.8227 mg/100 ml 0.0002, 239.4238 mg/100 ml 0.0002, 271.1457 mg/100 ml 0.0002 and 252.1662 mg/100 ml respectively. These concentrations were greater than that indicated on the packs which were 200 mg/100 ml for all the samples. When the method was tested with a prepared standard of concentration 0.2 mg/ml, the result obtained was 0.2068 mg/ml 0.0002 representing an increment of 3.4 % more than expected. Hence, the method could only account for 3.4 % of the increase in the sample concentration. The proposed method gave good validation results and the statistical analysis performed proved that the method is precise, accurate and reproducible and hence can be employed for routine analysis of ciprofloxacin in intravenous infusions.


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