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ARPN Journal of Science and Technology >> Volume 7, Issue 2, November 2017

ARPN Journal of Science and Technology


In Vitro Plant Regeneration from Shoot Tip Explants Of Jatropha Curcas L: A Biodiesel Plant

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Author Kamrun Nahar, Rita Sarah Borna
ISSN 2225-7217
On Pages 38-42
Volume No. 3
Issue No. 1
Issue Date February 01, 2013
Publishing Date February 01, 2013
Keywords Jatropha curcas, Shoot tip explants, Plant regeneration, Bidiesel plant, in vitro culture.



Abstract

In vitro regeneration was achieved from shoot tip explants of 4 months old Jatropha curcas plant. Propagation from shoot tip was evaluated on a range of concentrations of Benzyl adenine (BA) with indole-3-butyric acid (IBA) as 2.22 然/L BA and 4.9 然/L IBA, 1.11 然/M BA and 0.48 然/L IBA, 0.42 然/L BA and 0.46 然/L IBA and 0.44 然/L BA and 0.44 然/L IBA. Higher regeneration potential that direct adventitious shoot induction was recorded highest on MS medium with 2.22 然/L BA and 4.9 然/L IBA. Regenerated shoots then rooted on half strength MS medium supplemented with IBA (0.5然). Following simple hardening procedures, the in vitro raised plants were transferred to soil and grown to maturity in the field


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