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ARPN Journal of Science and Technology >> Volume 7, Issue 2, November 2017

ARPN Journal of Science and Technology


Evaluation of Different Botanical Plant Extracts and Other Material against Enset Bacterial Wilt (Xanthomonas campestris PV Musacearum) Disease in Oromia Regional State, Ethiopia

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Author Daniel Kasa, Getaneh Woldeab
ISSN 2225-7217
On Pages 68-73
Volume No. 5
Issue No. 2
Issue Date March 01, 2015
Publishing Date March 01, 2015
Keywords Enset bacterial wilt, Xanthomonas campestris pv. Musacearum, Botanical extract, Disk diffusion, Antibacterial activity



Abstract

Enset bacterial wilt (EBW) disease caused by Xanthomonas campestris pv. musacearum is a major constraint of Enset production in Toke-Kutaye District, Oromia Regional State. Farmer indigenous practices to controlling Enset bacterial wilt in the area is by using extracts of botanical plants which is recently becoming an important in development of new control measures. However, detailed study for identifying the potential botanicals against the disease was found necessary. Therefore, this study was conducted with the objective to evaluate the crud extracts botanicals, goat urine, and salt against the pathogen. In vitro, antibacterial activities of leaf and root extracts of the botanicals plant were examined against EBW isolates. The cruds extracts of botanicals such as such ‘Solle’ (Olinia rochetiana), ‘Hadafiti’ (Clematis simensis), ‘Tembosuse’ (Inula conferiflora), ‘Etecha’ (Dodonaea angustifolia), ‘Kebericho’ (Echinops kebericho), ‘goat urine’ and ‘salt’ individually and in combinations were tested using disc diffusion method. The results of vitro study showed that the inhibition zone (21.02mm) for the standard check (Penicillin) was significantly higher than all treatments under study. However, maximum growth inhibition zone 14.05mm was observed in the extract combination of Etecha + Kabericho followed by single extract of Etecha and combination of Solle + Hidafite + Tembosuse with inhibition zone of 12.08 and 11.78mm respectively. Results indicate the potential of these plants for further work on isolation and characterization of the active principle responsible for antibacterial activity and its exploitation as therapeutic agent.


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