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ARPN Journal of Science and Technology >> Volume 7, Issue 1, January 2017

ARPN Journal of Science and Technology


Effects of Cultural Conditions on the Production of Extracellular Protease by Streptomyces Albolongus and Streptomyces Aburaviensis

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Author Naznin Akhtar, Abu Sayeed Mohammad Mahmud, Muhammad Shahjalal Khan, Muhammad Ehteshamul Haque, Sharmin Sultana, M.N. Anwar
ISSN 2225-7217
On Pages 892-900
Volume No. 3
Issue No. 8
Issue Date September 01, 2013
Publishing Date September 01, 2013
Keywords Streptomyces albolongus, Streptomyces aburaviences, extracellular proteases, culture conditions



Abstract

Proteolytic activity of two isolates of actinomycetes Streptomyces albolongus and Streptomyces aburaviences was investigated on the basis of their ability to hydrolyze skimmed milk casein, egg albumin and gelatin. Both isolates were found to have potential for extracellular proteases production. Effects of culture conditions for the production of extracellular protease from S. albolongus and S. aburaviensis were determined. Highest protease yield from S. albolongus was obtained after 5 days of incubation with an initial pH 7, at static state when inoculated in medium composed of 1% glucose, 2% beef extract, 0.2% yeast extract, 0.1% KH2PO4, 0.3% K2HPO4, and trace MgSO4.7H2O. Optimum incubation conditions for S. abureviences were 4 days, with an initial medium pH 8 at shaking condition (100 rpm). S. aburaviences preferred 1.5% lactose and 1.5% tryptone as a carbon and nitrogen source. Both the isolates showed maximum protease yield at 370 C. The result of the present study might be helpful for large-scale production of extracellular protease from these Streptomyces spp.


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