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ARPN Journal of Science and Technology >> Volume 7, Issue 1, January 2017

ARPN Journal of Science and Technology


Diversity of Entophytic Bacteria in Symptomatic and Asymptomatic Infected Bananas from Endemic Area of Blood Disease Bacterium Based on RISA

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Author Hadiwiyono, Siti Subandiyah, Jaka Widada, Mark Fegan, Peter Taylor
ISSN 2225-7217
On Pages 376-381
Volume No. 3
Issue No. 4
Issue Date May 01, 2013
Publishing Date May 01, 2013
Keywords PCR, RISA, Blood Disease Bacterium, banana, entophytic bacterium.



Abstract

The relationship between the diversity of endophytic bacteria and disease development has been documented on many diseases. In Indonesia, by the millions banana are infected by Blood Disease Bacterium, causing severe damages. In this work, the diversity analysis of endophytic bacteria in infected bananas from endemic area of blood disease bacterium had been conducted using Ribosomal Intergenic Spacer Analysis (RISA). RISA is a Polymerase Chain Reaction (PCR)-based method as a useful assessment tool for the diversity analysis of microbes in the environment. Here, the infected bananas were determined by PCR using specific primer for Blood Disease Bacterium (BDB). Asymptomatic and symptomatic mats samples were analyzed through independent cultureable approach of PCR-RISA using universal primers of operon gene region of rRNA between small subunit (16S) and large subunit (23S) called intergenic spacer region (ISR). The diversity of endophytic bacteria from asymptomatic banana was different from that of symptomatic plants. In addition, the cultivar and habitat type might affect the diversity of endophytic bacteria.


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